MRM Optimization for Surrogate Peptides for LC-MS Protein Quantification

Paula Orens, Application Scientist, examines a typical workflow for surrogate peptide approach via LC-MS with tandem quadrupole mass spectrometry. Identifying transitions and optimizing MS conditions is a key focus of this module. By the end, you will understand how mass spectrometry informatics systesm combined with an open-source client MRM application can work together to simplify MRM method development. Using Infliximab as an example, Ms. Orens demonstrates the development and optimization of MRM transitions to establish sensitivity needs and discusses sample prep and LC considerations.

see "Peptide and Protein Quantification Boot Camp" for more training modules →