Small Molecule Bioanalysis
When searching for the hard-to-find molecule, gaining insights and expertise from the DMPK community is critical. Browse these resources to be even more effective in quantifying small molecules for drug efficacy.
When searching for the hard-to-find molecule, gaining insights and expertise from the DMPK community is critical. Browse these resources to be even more effective in quantifying small molecules for drug efficacy.
In this technology brief, a method for the analysis of serum cortisol, androstenedione, and 17-hydroxyprogesterone was successfully developed on an ACQUITY UPLC I-Class with 2D Technology with a Xevo TQD. A simple protein precipitation followed by separation using an ACQUITY UPLC BEH C18 SB VanGuard™ Pre-column in the first dimension and an ACQUITY UPLC HSS T3 Column in the second dimension was used to selectively clean the serum sample to provide optimum analytical performance.