Small Molecule Bioanalysis

When searching for the hard-to-find molecule, gaining insights and expertise from the DMPK community is critical. Browse these resources to be even more effective in quantifying small molecules for drug efficacy.

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Analysis of 17-Hydroxyprogesterone in Serum for Clinical Research

An analytically sensitive and selective clinical research method has been developed for the analysis of 17-OHP in serum. Using only 100 μL sample volume, this method provides sufficient analytical sensitivity to analyze low physiological levels of 17-OHP.

Quantitative Analysis of Dried Bloodspot 17-Hydroxyprogesterone by ACQUITY UPLC-MS/MS for Clinical Research

In this application note a method for the extraction and UPLC-MS/MS analysis of DBS 17-OHP using the ACQUITY UPLC System with the Xevo TQ MS Detector is described.

Analysis of Serum Cortisol, Androstenedione, and 17-hydroxyprogesterone Using 2D UPLC-MS/MS for Clinical Research

In this technology brief, a method for the analysis of serum cortisol, androstenedione, and 17-hydroxyprogesterone was successfully developed on an ACQUITY UPLC I-Class with 2D Technology with a Xevo TQD. A simple protein precipitation followed by separation using an ACQUITY UPLC BEH C18 SB VanGuard™ Pre-column in the first dimension and an ACQUITY UPLC HSS T3 Column in the second dimension was used to selectively clean the serum sample to provide optimum analytical performance.

Analysis of Testosterone Using the Xevo TQ-XS for Clinical Research

This technology brief demonstrates the high analytical sensitivity and quantitative performance of the Xevo® TQ-XS for the analysis of serum testosterone for clinical research using 100 μL serum.

Analysis of Plasma 17-Hydroxyprogesterone, Androstenedione, and Cortisol

Sample preparation is an important consideration for any bioanalytical LC-MS/MS method for clinical research. Waters has developed a novel sample preparation sorbent, Oasis® PRiME HLB, which is designed to have some key advantages over traditional SPE sorbents.

Analysis of Corticosteroids and Androgens in Serum for Clinical Research

An analytically sensitive and selective clinical research method has been developed for the analysis of testosterone, androstenedione, 17-OHP, DHEAS, cortisol, 11-deoxycortisol, and 21-deoxycortisol in serum using the Xevo TQ-S micro.

Top ten misconceptions about microflow LC/MS

You’ve heard a lot about microflow LC/MS. Are you cautious about making the switch? Do you still have questions or concerns about method development? We are going to use this time to review the top 10 misconceptions and challenges that we have heard and observed. Our hope is that after you have listened to this presentation, you will be able to make an informed decision on how microflow LC/MS will fit into your workflow.

The Versatility of Microflow LC/MS – Making High Sensitivity Applications More Routine with ionKey/MS

Originally launched in response to the need for robust, high sensitivity microflow LC/MS applications, the integrated microfluidic tile-based ionKey/MS™ platform continues to evolve as a system that can be implemented for routine applications. ionKey/MS™ has quickly gained traction in new application spaces yielding a more functional and flexible system. These new areas include quantification of intact and digested monoclonal antibodies, thyroglobulin, opioids in oral fluid, estradiol, and steroids.

Peer reviewed literature for microflow LC-MS
August 2017

The following is a compendium of microflow LC-MS-based peer reviewed journal articles using Waters® ionKey/MS™ System. Each article is authored by leading researchers and/or Waters’ scientists. They cover many analytes of interest including small and large molecule therapeutics, biomarkers, supplements, and food.

Peer reviewed literature for microflow LC-MS
August 2017

The following is a compendium of microflow LC-MS-based peer reviewed journal articles using Waters® ionKey/MS™ System. Each article is authored by leading researchers and/or Waters’ scientists. They cover many analytes of interest including small and large molecule therapeutics, biomarkers, supplements, and food.