Metabolite Identification
Characterize and quantify metabolites with speed, efficiency, and confidence.
This data demonstrates the advantage of ion mobility separation combined with DIA for DMPK studies. Use of this orthogonal separation technique is capable of significantly improving the confidence and speed with which drug metabolism and degradation products can be identified. The dextromethorphan metabolite 2x + O product ion signals are relatively minor when compared to interfering matrix ions, these are not separated using traditional LC-MS analysis and therefore can complicate assignment. When this same data is analyzed incorporating the ion mobility dimension, matrix product ions are removed, and therefore, metabolite product ions are easily identified and confidently assigned.
A unique challenge in peptide-based drug metabolism for evaluating preclinical candidates is the need for processing and visualization software tools that accommodate both small molecule rules (such as oxidations, synthetic modifications), as well as large molecule modifications (such as amide hydrolysis and oxidative deamination).