Metabolite Identification

Characterize and quantify metabolites with speed, efficiency, and confidence.

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Improving Drug Metabolite Identification in Biofluids with the ACQUITY PREMIER and Hybrid Organic Surface Technology: Increased Sensitivity and Reproducibility
February 2021

In this application note we demonstrate the benefit of using the ACQUITY PREMIER UPLC Chromatography System for the analysis of metabolites of gefitinib in mouse urine.

Ion Mobility for Metabolite Characterization: Improving Spectral Clarity of Data Independent Acquisitions
January 2021

This data demonstrates the advantage of ion mobility separation combined with DIA for DMPK studies. Use of this orthogonal separation technique is capable of significantly improving the confidence and speed with which drug metabolism and degradation products can be identified. The dextromethorphan metabolite 2x + O product ion signals are relatively minor when compared to interfering matrix ions, these are not separated using traditional LC-MS analysis and therefore can complicate assignment. When this same data is analyzed incorporating the ion mobility dimension, matrix product ions are removed, and therefore, metabolite product ions are easily identified and confidently assigned.

Ion Mobility-Enabled Metabolite Identification of Tienilic Acid and Tienilic Acid Isomer Using Mass-MetaSite and WebMetabase

In this application note we have demonstrated the coupling of HRMS with an additional, ion mobility-based, gas-phase separation for the determination of metabolic profiles of tienilic acid (TA) and its 3-thiophene isomer.

Metabolite Identification of Complex Cyclic Peptides Using WebMetabase, Ion Mobility-Enabled DIA, and Product Ion Confirmation

In this application note, we describe a solution that uses an information-rich data acquisition mode, which effectively deals with the complexity of modified peptides.

Ion mobility spectrometry: The future of pharma analysis

Check out our article to discover how IMS technology is driving pharmaceutical research forward, from identifying toxic metabolites across the drug development pipeline to explaining possible causes of Alzheimer’s disease.

Somatostatin Analogue Catabolite Screening and Identification Using Vion IMS QTof with WebMetabase

In this application note, several serum samples were analyzed using ion mobility enabled high resolution mass spectrometry.

Characterization of Differential Metabolites of Tienilic Acid and Its 3-Thiophene Isomer with Ion Mobility Enabled Mass Spectrometry

Tienilic acid (TA) is a uricosuric diuretic found to induce immune-mediated hepatotoxicity in patients, while its 3-thiophene isomer (TAI) exhibits direct hepatotoxic effects, and differential metabolism has been reported for these two compounds.

Routine Metabolite Identification for Complex Peptides Based on IMS Enabled QTof DIA Data Acquisition and Mass-Metasite Data Processing

Ion mobility enabled non-targeted HDMSE acquisition coupled with Mass-MetaSite and WebMetabase provides seamless data acquisition and processing for routine metabolite determination of complex cyclic peptides.

Taking Advantage of Ion Mobility Enabled Approaches for Discovery Metabolite Identification

This on-demand webinar will show you how ion mobility spectrometry and collision cross-section can increase your confidence in metabolite identification and structural identification for drug discovery, and make your data interpretation easier.

Improving Peptide Catabolism Interpretation Using Ion Mobility Data and Server-Based Data Review With HELM Integration

A unique challenge in peptide-based drug metabolism for evaluating preclinical candidates is the need for processing and visualization software tools that accommodate both small molecule rules (such as oxidations, synthetic modifications), as well as large molecule modifications (such as amide hydrolysis and oxidative deamination).