Large Molecule Quantification

Address the quantification of peptide- and protein-based biotherapeutics and biomarkers.

Lost Samples in the Container: Non-specific Binding and the Impact of Blocking Agents

My sample stayed in the solution…or did it? Samples are precious. Well-prepared samples are the starting point of all successful experiments. That’s why we spend time and effort on sample preparation; so that the resulting samples are ready for downstream LC analyses. When we think of good sample prep and handling, we emphasize the sample purity, concentration, volume, etc. but often overlook the importance of sample recovery during storage. In fact, it is not uncommon for a perfectly well-prepared sample to be lost on the surface of a sample container while it is waiting for injection.

Where Did My Sample Go?

A case of non-specific binding (NSB). We’ve all been there. You spend hours (sometimes days) in the lab preparing your samples. You carefully walk them to the autosampler, lovingly load up your sample queue, and press play. Of course you’re running these overnight because instrument time is precious and someone else needs to use it tomorrow. You come into the lab in the morning and immediately get settled in to process your data. And there’s nothing there....

Tackling Non-Specific Binding of Biotherapeutics Using LC-MS Compatible QuanRecovery Sample Plates with MaxPeak High Performance Surfaces

This technical note demonstrates the ability of QuanRecovery Sample Plates with MaxPeak HPS (High Performance Surfaces) to mitigate non-specific binding of monoclonal antibody biotherapeutics and improve analytical method performance.

Automating Sample Preparation Workflows for Hybrid LC-MS/MS Bioanalysis of Protein Therapeutics: Quantification of Etanercept Using Affinity Purification and Digestion

This application note aims to describe the process of developing a successful, automated capture and digestion method for hybrid LC-MS/MS quantification of the fusion protein, etanercept.

Development of a Hybrid Immunoaffinity-LC-MS/MS Method for the Quantification of Active Biotherapeutics Targeting TNF-α in Serum

This application note presents a highly sensitive and selective sample preparation strategy for the LC-MS/MS quantification of free/active biotherapeutic from human serum for the TNF-α targeting biotherapeutics: infliximab, adalimumab, and etanercept.

High Sensitivity Quantification of Salmon Calcitonin from Human Serum Using Mixed-Mode SPE & LC-MS/MS

In this work, we have a developed a complete sample preparation and LC-MS/MS workflow for the sensitive and accurate quantification of salmon calcitonin from human serum.

An Intact Level Workflow for Sensitive Monoclonal Antibody Monitoring in Preclinical Matrix

This poster demonstrates HRMS sensitivity of intact level quantification for three monoclonal antibodies, adalimumab, infliximab, and trastuzumab prepared through affinity purification in mouse plasma.

Development of a SPE LC-MS/MS Method for the Bioanalytical Quantification of Salmon Calcitonin from Human Serum

This application note describes a simple sample preparation strategy using Oasis WCX μElution SPE combined with analytical flow LC and a tandem (triple) quadrupole MS for the high sensitivity quantification of salmon calcitonin from human serum.

Oasis Peptide Bioanalysis Reference Card

This reference card highlights bioanalytical sample preparation and method development for therapeutic and endogenous peptides.

High Resolution Mass Spectrometry for Quantitative and Qualitative (Quant/Qual) Bioanalysis of Antibody-based Therapeutics using Intact mAb and Subunit Detection

John T. Mehl, Ph.D Sr. Research Investigator, Bioanalytical Research Bristol-Myers Squibb

As antibody-based therapeutics become more common and more structurally complex, there is an increased need for more in depth understanding of the in vivo disposition of this class of drug modality. This presentation provides an overview of affinity sample preparation and highlight examples of how both Intact and subunit mAb bioanalysis provides insight into the in vivo disposition of antibody-based therapeutics.