Large Molecule Quantification
Address the quantification of peptide- and protein-based biotherapeutics and biomarkers.
Address the quantification of peptide- and protein-based biotherapeutics and biomarkers.
My sample stayed in the solution…or did it? Samples are precious. Well-prepared samples are the starting point of all successful experiments. That’s why we spend time and effort on sample preparation; so that the resulting samples are ready for downstream LC analyses. When we think of good sample prep and handling, we emphasize the sample purity, concentration, volume, etc. but often overlook the importance of sample recovery during storage. In fact, it is not uncommon for a perfectly well-prepared sample to be lost on the surface of a sample container while it is waiting for injection.
A case of non-specific binding (NSB). We’ve all been there. You spend hours (sometimes days) in the lab preparing your samples. You carefully walk them to the autosampler, lovingly load up your sample queue, and press play. Of course you’re running these overnight because instrument time is precious and someone else needs to use it tomorrow. You come into the lab in the morning and immediately get settled in to process your data. And there’s nothing there....
John T. Mehl, Ph.D Sr. Research Investigator, Bioanalytical Research Bristol-Myers Squibb
As antibody-based therapeutics become more common and more structurally complex, there is an increased need for more in depth understanding of the in vivo disposition of this class of drug modality. This presentation provides an overview of affinity sample preparation and highlight examples of how both Intact and subunit mAb bioanalysis provides insight into the in vivo disposition of antibody-based therapeutics.