Large Molecule Quantification

Address the quantification of peptide- and protein-based biotherapeutics and biomarkers.

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LC-MS/MS Quantification of Intact Insulin-Like Growth Factor I (IGF-I) from Serum for Clinical Research

This poster was presented at the 2018 ASMS annual conference from June 3-7 in San Diego, CA.

Peptide Catabolite Identification Using HDMSE data and Mass-MetaSite Processing

This poster was presented at the 2018 ASMS annual conference from June 3-7 in San Diego, CA.

Evaluation of Intelligent Software Tools for the Metabolite Profiling and Identification of Peptide-Based Large Molecules

This poster was presented at the 2018 ASMS annual conference from June 3-7 in San Diego, CA.

Development of A Hybrid Immunoaffinity-LC-MS/MS Method for the Quantification of Active Biotherapeutics Targeting TNF- α In Serum

This poster was presented at the 2018 ASMS annual conference from June 3-7 in San Diego, CA.

Analyzing Data Processing Parameters for HRMS-Based Intact Level mAb Quantification

This poster was presented at the 2018 ASMS annual conference from June 3-7 in San Diego, CA.

Characterizing the Catabolism of Peptides Using Ion Mobility Enabled High Resolution Mass Spectrometry

This poster was presented at the 2018 ASMS annual conference from June 3-7 in San Diego, CA.

High Sensitivity Intact Monoclonal Antibody (mAb) HRMS Quantification

The complexity of trastuzumab, and biotherapeutic proteins in general, require a multifaceted and flexible approach for their characterization and quantification. Highly sensitive direct quantitation at the intact level was achieved using a Waters Vion IMS QTof and UNIFI.

Accurate and Sensitive LC-MS/MS Quantification of Adalimumab in Serum/Plasma: Impact of Sample Preparation on Method Performance

This application note describes the sensitive and selective quantification of adalimumab from serum/plasma using a kit-based approach for sample digestion and peptide level clean-up. Coupling different enrichment techniques to the standardized approach of ProteinWorks eXpress Digest and SPE Clean-up Kits provided substantial sensitivity benefit with the increased complexity of each technique.

Comparison of Tandem and High Resolution Mass Spectrometry for the Quantification of the Monoclonal Antibody, Trastuzumab in Plasma

This technology brief demonstrates the sensitive and robust HRMS quantification of trastuzumab from plasma.

Accurate Quantification of intact Insulin-like Growth Factor I (IGF-I) using High Resolution Mass Spectrometry (HRMS) for Clinical Research

This technology brief demonstrates analytically sensitive, accurate, and robust quantification of intact IGF-I in biological matrix using a simple sample preparation workflow, an analytical LC and HRMS.

HRMS platforms, with their ability to provide qualitative and quantitative information offer a flexible alternative to TQ systems, especially for direct quantification of intact proteins. With improvements in hardware and software, HRMS platforms can now achieve the performance characteristics required for bioanalytical quantification, as highlighted by the analytically sensitive (10 ng/mL) and robust (CV’s <10%) quantification of intact IGF-I for clinical research.