Large Molecule Quantification

Address the quantification of peptide- and protein-based biotherapeutics and biomarkers.

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Achieving Maximum Protein and Peptide Recovery, Sensitivity, and Reproducibility using QuanRecovery Vials and Plates

Quantitative LC-MS analyses for proteins and peptides are getting more challenging every day, requiring greater sensitivity and reproducibility from smaller amounts of samples. Analyte loss due to non-specific binding in sample containers is a significant problem in quantitation that is often not recognized early enough.

LC-MS Analysis of Plasma from Patients with Insulin Autoimmune Syndrome

This presentation was done at the 2019 ASMS Waters MS Users Meeting Pharmaceutical Track by: Dr Richard Kay, Cambridge University

Tandem Quadrupole MS for The Quantification of Monoclonal Antibody Subunit Light Chains In Plasma

Quantification of mAb subunit light chains via tandem quadrupole MS, achieving 25 ng/mL LLOQs from plasma.

Development of a SPE LC-MS/MS Method Utilizing QuanRecovery Sample Plates with MaxPeak Performance Surfaces for the Bioanalytical Quantification of Pramlintide from Serum

This work describes a selective sample preparation strategy and LC-MS compatible sample storage plates with high performance surfaces to mitigate pramlintide loss due to non-specific binding. In addition, it shows that optimized UPLC separation and column chemistry, along with a highly sensitive tandem quadrupole mass spectrometry method, can produce significantly lower limits of quantification.

Factors That Influence the Recovery Of Hydrophobic Peptides During LC-MS Sample Handling

We reviewed how various factors that influenced the peptide loss and proposed a systematic method to maximize the recovery.

Rapid, Sensitive, and Routine Intact mAb Quantification using a Compact Tof HRMS Platform

Compact, simple and sensitive HRMS platform with improved data quality evaluated for intact level mAb quantitation in plasma.

What do great vacations and peptide bioanalysis have in common

For starters no one wants a disastrous vacation, the same goes for a bioanalysis assay! What makes for a great vacation? A booking agent who understands you, on time transit, a room with a view a stones throw from the beach, friendly staff and great cuisine?

Sample Prep before LC-MS Quantification of Peptides and Proteins

Confronting the challenge of sensitivity and sample loss. As a relative newcomer to peptide and protein quantification coming from the small molecule world, I have learned that I need to think differently, and more creatively, to meet the challenges of these molecules. As scientists with assay sensitivity goals, we sometimes have to take extra steps to ensure that we not only meet this goal, but we also ensure reproducibility and overall robustness of the methods we develop. As I think about this challenge, I find it helpful to think of the workflow from the perspective of my sample.

Lost Samples in the Container: Non-specific Binding and the Impact of Blocking Agents

My sample stayed in the solution…or did it? Samples are precious. Well-prepared samples are the starting point of all successful experiments. That’s why we spend time and effort on sample preparation; so that the resulting samples are ready for downstream LC analyses. When we think of good sample prep and handling, we emphasize the sample purity, concentration, volume, etc. but often overlook the importance of sample recovery during storage. In fact, it is not uncommon for a perfectly well-prepared sample to be lost on the surface of a sample container while it is waiting for injection.

Where Did My Sample Go?

A case of non-specific binding (NSB). We’ve all been there. You spend hours (sometimes days) in the lab preparing your samples. You carefully walk them to the autosampler, lovingly load up your sample queue, and press play. Of course you’re running these overnight because instrument time is precious and someone else needs to use it tomorrow. You come into the lab in the morning and immediately get settled in to process your data. And there’s nothing there....