Large Molecule Quantification

Address the quantification of peptide- and protein-based biotherapeutics and biomarkers.

Development of a SPE LC-MS/MS Method Utilizing QuanRecovery Sample Plates with MaxPeak Performance Surfaces for the Bioanalytical Quantification of Pramlintide from Serum

This work describes a selective sample preparation strategy and LC-MS compatible sample storage plates with high performance surfaces to mitigate pramlintide loss due to non-specific binding. In addition, it shows that optimized UPLC separation and column chemistry, along with a highly sensitive tandem quadrupole mass spectrometry method, can produce significantly lower limits of quantification.

Factors That Influence the Recovery Of Hydrophobic Peptides During LC-MS Sample Handling

We reviewed how various factors that influenced the peptide loss and proposed a systematic method to maximize the recovery.

Rapid, Sensitive, and Routine Intact mAb Quantification using a Compact Tof HRMS Platform

Compact, simple and sensitive HRMS platform with improved data quality evaluated for intact level mAb quantitation in plasma.

What do great vacations and peptide bioanalysis have in common

For starters no one wants a disastrous vacation, the same goes for a bioanalysis assay! What makes for a great vacation? A booking agent who understands you, on time transit, a room with a view a stones throw from the beach, friendly staff and great cuisine?

Sample Prep before LC-MS Quantification of Peptides and Proteins

Confronting the challenge of sensitivity and sample loss. As a relative newcomer to peptide and protein quantification coming from the small molecule world, I have learned that I need to think differently, and more creatively, to meet the challenges of these molecules. As scientists with assay sensitivity goals, we sometimes have to take extra steps to ensure that we not only meet this goal, but we also ensure reproducibility and overall robustness of the methods we develop. As I think about this challenge, I find it helpful to think of the workflow from the perspective of my sample.

Lost Samples in the Container: Non-specific Binding and the Impact of Blocking Agents

My sample stayed in the solution…or did it? Samples are precious. Well-prepared samples are the starting point of all successful experiments. That’s why we spend time and effort on sample preparation; so that the resulting samples are ready for downstream LC analyses. When we think of good sample prep and handling, we emphasize the sample purity, concentration, volume, etc. but often overlook the importance of sample recovery during storage. In fact, it is not uncommon for a perfectly well-prepared sample to be lost on the surface of a sample container while it is waiting for injection.

Where Did My Sample Go?

A case of non-specific binding (NSB). We’ve all been there. You spend hours (sometimes days) in the lab preparing your samples. You carefully walk them to the autosampler, lovingly load up your sample queue, and press play. Of course you’re running these overnight because instrument time is precious and someone else needs to use it tomorrow. You come into the lab in the morning and immediately get settled in to process your data. And there’s nothing there....

Tackling Non-Specific Binding of Biotherapeutics Using LC-MS Compatible QuanRecovery Sample Plates with MaxPeak High Performance Surfaces

This technical note demonstrates the ability of QuanRecovery Sample Plates with MaxPeak HPS (High Performance Surfaces) to mitigate non-specific binding of monoclonal antibody biotherapeutics and improve analytical method performance.

Automating Sample Preparation Workflows for Hybrid LC-MS/MS Bioanalysis of Protein Therapeutics: Quantification of Etanercept Using Affinity Purification and Digestion

This application note aims to describe the process of developing a successful, automated capture and digestion method for hybrid LC-MS/MS quantification of the fusion protein, etanercept.

Development of a Hybrid Immunoaffinity-LC-MS/MS Method for the Quantification of Active Biotherapeutics Targeting TNF-α in Serum

This application note presents a highly sensitive and selective sample preparation strategy for the LC-MS/MS quantification of free/active biotherapeutic from human serum for the TNF-α targeting biotherapeutics: infliximab, adalimumab, and etanercept.