Large Molecule Quantification

Address the quantification of peptide- and protein-based biotherapeutics and biomarkers.

Automating Sample Preparation Workflows for Hybrid LC-MS/MS Bioanalysis of Protein Therapeutics: Quantification of Etanercept Using Affinity Purification and Digestion

This application note aims to describe the process of developing a successful, automated capture and digestion method for hybrid LC-MS/MS quantification of the fusion protein, etanercept.

Development of a Hybrid Immunoaffinity-LC-MS/MS Method for the Quantification of Active Biotherapeutics Targeting TNF-α in Serum

This application note presents a highly sensitive and selective sample preparation strategy for the LC-MS/MS quantification of free/active biotherapeutic from human serum for the TNF-α targeting biotherapeutics: infliximab, adalimumab, and etanercept.

High Sensitivity Quantification of Salmon Calcitonin from Human Serum Using Mixed-Mode SPE & LC-MS/MS

In this work, we have a developed a complete sample preparation and LC-MS/MS workflow for the sensitive and accurate quantification of salmon calcitonin from human serum.

An Intact Level Workflow for Sensitive Monoclonal Antibody Monitoring in Preclinical Matrix

This poster demonstrates HRMS sensitivity of intact level quantification for three monoclonal antibodies, adalimumab, infliximab, and trastuzumab prepared through affinity purification in mouse plasma.

Development of a SPE LC-MS/MS Method for the Bioanalytical Quantification of Salmon Calcitonin from Human Serum

This application note describes a simple sample preparation strategy using Oasis WCX μElution SPE combined with analytical flow LC and a tandem (triple) quadrupole MS for the high sensitivity quantification of salmon calcitonin from human serum.

Oasis Peptide Bioanalysis Reference Card

This reference card highlights bioanalytical sample preparation and method development for therapeutic and endogenous peptides.

High Resolution Mass Spectrometry for Quantitative and Qualitative (Quant/Qual) Bioanalysis of Antibody-based Therapeutics using Intact mAb and Subunit Detection

John T. Mehl, Ph.D Sr. Research Investigator, Bioanalytical Research Bristol-Myers Squibb

As antibody-based therapeutics become more common and more structurally complex, there is an increased need for more in depth understanding of the in vivo disposition of this class of drug modality. This presentation provides an overview of affinity sample preparation and highlight examples of how both Intact and subunit mAb bioanalysis provides insight into the in vivo disposition of antibody-based therapeutics.

Developing bioanalytical assays and workflows for peptide/protein biomarkers and biotherapeutics: Getting the right assay the first time

Matt Szapacs, Ph.D Group Leader Exploratory Biomarker Assay Group GlaxoSmithKline

In recent years the use of LC-MS technologies for the quantitation of peptide/protein biomarkers has increased dramatically. This presentation focuses on case studies highlighting the use of systematic method development strategies for both intact analysis and methods involving digestion. In addition, instrument selection (High resolution vs. QQQ) will be highlighted.

A Powerful Hybrid IA-LC-MS/MS Method for Routine Quantification of Insulin Analogues and their Biosimilars in Clinical Trial Samples

Michael Blackburn, M.Phil Bioanalytical Scientist, Method Development Specialist ARCINOVA

Mass spectrometry based assays are rapidly becoming the gold standard for the analysis of insulin biosimilars, and are increasingly requested by regulatory authorities during clinical trials for new diabetic therapies, due to their superior specificity. In this presentation, we show how the sensitivity of the Xevo TQ-XS combined with immunoaffinity extraction and UPLC enables bioanalysts to bring new compounds to market.

A Guide to Performing Method Development for Direct Injection Methods Using ionKey/MS in a Bioanalytical Laboratory: From Sample Preparation to LC-MS

This white paper describes a suggested approach to developing methods for use with the ionKey/MS System. The instrumentation may consist of the ACQUITY UPLC M-Class System, and any Xevo/SYNAPT Mass Spectrometer that can be fitted with an ionKey source.