Large Molecule Quantification

Address the quantification of peptide- and protein-based biotherapeutics and biomarkers.

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More sensitivity is always better: Measuring sub-clinical levels of serum thyroglobulin on a µLC–MS/MS system

Although liquid chromatography-tandem mass spectrometry (LC-MS/MS) assays for thyroglobulin (Tg) are resistant to autoantibody (TgAb) interference, recent studies have demonstrated approximately 40% of TgAb-positive individuals with recurrent thyroid cancer have Tg concentrations below the lower limit of quantification (LLOQ) of the LC-MS/MS assays described to date (i.e., <0.5 ng/mL), resulting in false-negative findings during post-thyroidectomy monitoring. To reduce false negative results due to insufficient analytical sensitivity, a new Tg assay was developed on a commercially available LC-MS/MS system operating at microliter/minute flow-rates (i.e., µLC–MS/MS) to maximize the analytical sensitivity and achieve a LLOQ of 0.02 ng/mL.

Microflow UPLC and high-resolution MS as a sensitive and robust platform for quantitation of intact peptide hormones

Recent advances in microflow ultra performance liquid chromatography (UPLC) systems offer higher sensitivity with robustness to meet the routine bioanalytical demands. Modern high-resolution mass spectrometers (HRMS) enable the development of highly selective methods with broad dynamic range.

Development of an UPLC/MS–MS method for quantification of intact IGF-I from human serum

Developing LC–MS methods for biomolecules is often challenging due to issues with molecular size and complexity, nonspecific binding, protein binding, solubility and sensitivity. As a result, complex sample preparation workflows, including immune-affinity and/or protein digestion and lengthy analysis potentially using nano-flow LC, may be needed to achieve the required sensitivity. This work aims to provide a simple, sensitive, fast and robust method for quantification of intact IGF-I from human serum using UPLC–MS/MS.

Triple Quadrupole Mass Spectrometry (Xevo TQ-XS) for the Quantification of Monoclonal Antibody Light Chains in Plasma

The work herein describes the development and optimization of sample preparation and LC-MS/MS methodology for the sensitive quantification of mAb subunit light chains using selective column chemistry and triple quadrupole mass spectrometry.

Peptide Bioanalysis Infographic

Peptide analysis can be challenging; but, with the right tools and steps, you can become a Peptide Pro. This infographic details A Four-Step Workflow for Peptide Bioanalysis.

Achieving Maximum Protein and Peptide Recovery, Sensitivity, and Reproducibility using QuanRecovery Vials and Plates

Quantitative LC-MS analyses for proteins and peptides are getting more challenging every day, requiring greater sensitivity and reproducibility from smaller amounts of samples. Analyte loss due to non-specific binding in sample containers is a significant problem in quantitation that is often not recognized early enough.

LC-MS Analysis of Plasma from Patients with Insulin Autoimmune Syndrome

This presentation was done at the 2019 ASMS Waters MS Users Meeting Pharmaceutical Track by: Dr Richard Kay, Cambridge University

Tackling Non-Specific Binding of Biotherapeutics Using LC-MS Compatible QuanRecovery Sample Plates with MaxPeak High Performance Surfaces

This technical note demonstrates the ability of QuanRecovery Sample Plates with MaxPeak HPS (High Performance Surfaces) to mitigate non-specific binding of monoclonal antibody biotherapeutics and improve analytical method performance.

Automating Sample Preparation Workflows for Hybrid LC-MS/MS Bioanalysis of Protein Therapeutics: Quantification of Etanercept Using Affinity Purification and Digestion

This application note aims to describe the process of developing a successful, automated capture and digestion method for hybrid LC-MS/MS quantification of the fusion protein, etanercept.

Development of a Hybrid Immunoaffinity-LC-MS/MS Method for the Quantification of Active Biotherapeutics Targeting TNF-α in Serum

This application note presents a highly sensitive and selective sample preparation strategy for the LC-MS/MS quantification of free/active biotherapeutic from human serum for the TNF-α targeting biotherapeutics: infliximab, adalimumab, and etanercept.