Peptide and Protein Bioanalysis Boot Camp

Building Proficiency in LC-MS Large Molecule Quantification

Peptide and Protein Bioanalysis Boot Camp

Waters’ team of bioanalysis experts developed the Peptide & Protein Bioanalysis Boot Camp – a series of short, recorded presentations – to help you and your colleagues acquire a fundamental and practical proficiency in large molecule quantification.

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Peptide Sample Preparation Optimization and Troubleshooting

Mass spectrometry, chromatography, and sample preparation are all leveraged to facilitate the development of sensitive, selective, and robust bioanalytical methods for the quantification of peptides in biological matrices. In this module, Erin Chambers, Vice President, Chemistry and Technology, focuses on peptide sample prep optimization and the fundamental characteristics of peptides.
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Identification of Surrogate Peptides for LC-MS Protein Quantification

In this module Nikunj Tanna, Waters' Senior Scientist, discusses the circumstances in which it's necessary to perform a surrogate peptide approach for protein quantification; the differences between generic and unique surrogate peptides; and he outlines various steps in identifying peptides, and the available software tools to aid the process.
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MRM Optimization for Surrogate Peptides for LC-MS Protein Quantification

Paula Orens, Application Scientist, examines a typical workflow for surrogate peptide approach via LC-MS with tandem quadrupole mass spectrometry. Identifying transitions and optimizing MS conditions is a key focus of this module. By the end, you will understand how mass spectrometry informatics systesm combined with an open-source client MRM application can work together to simplify MRM method development. Using Infliximab as an example, Ms. Orens demonstrates the development and optimization of MRM transitions to establish sensitivity needs and discusses sample prep and LC considerations.
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Protein Level Sample Purification and Digestion for LC-MS Quantification

Waters Product Manager, Steven Calciano, discusses the bioanalytical challenges of protein level sample purification and digestion.
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Internal Standard Options for Peptide LC-MS Quantification

An internal standard is a compound that displays physical and chemical characteristics similar to that of the analyte of interest, but at the same time generates a response that can be distinguished from that of the analyte. In Part 1 of "Internal Standard (IS) Options," Erin Chambers focuses on Peptide LC-MS quantification. She discusses various IS options when, where, and how to implement various strategies, and accurate peptide quantification with various options.
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Internal Standard Options for Protein LC-MS Quantification

In Part 2 of "Internal Standard (IS) Options," Erin Chambers focuses on Protein LC-MS quantification. She discusses when, where, and how to implement various strategies and accurate protein quantification with various IS options.
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