Peptide and Protein Bioanalysis Boot Camp
Building Proficiency in LC-MS Large Molecule Quantification
Waters’ team of bioanalysis experts developed the Peptide & Protein Bioanalysis Boot Camp – a series of short, recorded presentations – to help you and your colleagues acquire a fundamental and practical proficiency in large molecule quantification.
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Peptide Sample Preparation Optimization and Troubleshooting
Mass spectrometry, chromatography, and sample preparation are all leveraged to facilitate the development of sensitive, selective, and robust bioanalytical methods for the quantification of peptides in biological matrices. In this module, Erin Chambers, Vice President, Chemistry and Technology, focuses on peptide sample prep optimization and the fundamental characteristics of peptides.
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As a principal scientist and now vice president of chemistry, Erin has worked at Waters for more than 14 years and has been in bioanalysis for almost 25. While at Yale, Erin recalls a conversation with her college dean that changed the course of her career and put her on the path of scientific study. Ultimately, she graduated from Yale University with a degree in chemistry focusing on molecular biochemistry and biophysics, and earned her doctoral degree from Kings College London. Erin loves the dynamic nature of her job, developing new extraction methods, and helping to design new analytical systems that have a profound influence on illnesses like diabetes and Alzheimer’s disease. She loves to play with her kids and being outdoors. Her favorite authors are Lincoln Child and Douglas Preston.
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Identification of Surrogate Peptides for LC-MS Protein Quantification
In this module Nikunj Tanna, Waters' Senior Scientist, discusses the circumstances in which it's necessary to perform a surrogate peptide approach for protein quantification; the differences between generic and unique surrogate peptides; and he outlines various steps in identifying peptides, and the available software tools to aid the process.
As a senior scientist, Nikunj Tanna is responsible for developing applications to support bioanalysis of large molecules, including therapeutic monoclonal antibodies, ADC’s, proteins and peptides as well as biomarkers. His past experience included method development and validation under GLP/CLIA guidelines for pharmacokinetic study of small molecule drugs, endogenous molecules for CPT code tests, and immunoaffinity extraction of protein biomarkers. He also led the routine analysis & data review of clinical samples in support of pharmacokinetics study and oversaw experimental design and data review for analytical and clinical validation of ELISA assays used in biomarker panels.
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MRM Optimization for Surrogate Peptides for LC-MS Protein Quantification
Paula Orens, Application Scientist, examines a typical workflow for surrogate peptide approach via LC-MS with tandem quadrupole mass spectrometry. Identifying transitions and optimizing MS conditions is a key focus of this module. By the end, you will understand how mass spectrometry informatics systesm combined with an open-source client MRM application can work together to simplify MRM method development. Using Infliximab as an example, Ms. Orens demonstrates the development and optimization of MRM transitions to establish sensitivity needs and discusses sample prep and LC considerations.
Paula began her career at a contract research organization supporting the high-throughput bioanalysis of small molecules and peptides. When she joined Waters in 2015, she transitioned into an Applications Scientist role, focused on new product development and discovery bioanalysis of peptides and proteins. With the ever changing and advancing Waters’ technologies, Paula’s kept busy with method development of therapeutic peptides and proteins.
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Protein Level Sample Purification and Digestion for LC-MS Quantification
Waters Product Manager, Steven Calciano, discusses the bioanalytical challenges of protein level sample purification and digestion.
Steven Calciano joined Waters Corporation in 2015 and is responsible bringing innovative large molecule chromatography and mass spectrometry sample preparation solutions to market. Prior to his current role, Steven supported analytical standards and reagents products across pharmaceutical, environmental, chemical and food industries, gaining an understanding of a variety of complex analytical workflows. He strives to bring enabling technology to LC-MS users which simplify complex sample preparation methods.
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Internal Standard Options for Peptide LC-MS Quantification
An internal standard is a compound that displays physical and chemical characteristics similar to that of the analyte of interest, but at the same time generates a response that can be distinguished from that of the analyte. In Part 1 of "Internal Standard (IS) Options," Erin Chambers focuses on Peptide LC-MS quantification. She discusses various IS options when, where, and how to implement various strategies, and accurate peptide quantification with various options.
As a Principal Scientist and now Vice President, Chemistry and Technology, Erin has worked at Waters for more than 14 years and has been in bioanalysis for almost 25. While at Yale, Erin recalls a conversation with her college dean that changed the course of her career and put her on the path of scientific study. Ultimately, she graduated from Yale University with a degree in chemistry focusing on molecular biochemistry and biophysics, and earned her doctoral degree from Kings College London. Erin loves the dynamic nature of her job, developing new extraction methods, and helping to design new analytical systems that have a profound influence on illnesses like diabetes and Alzheimer’s disease. She loves to play with her kids and being outdoors. Her favorite authors are Lincoln Child and Douglas Preston.
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Internal Standard Options for Protein LC-MS Quantification
In Part 2 of "Internal Standard (IS) Options," Erin Chambers focuses on Protein LC-MS quantification. She discusses when, where, and how to implement various strategies and accurate protein quantification with various IS options.
As a Principal Scientist and now Vice President, Chemistry and Technology, Erin has worked at Waters for more than 14 years and has been in bioanalysis for almost 25. While at Yale, Erin recalls a conversation with her college dean that changed the course of her career and put her on the path of scientific study. Ultimately, she graduated from Yale University with a degree in chemistry focusing on molecular biochemistry and biophysics, and earned her doctoral degree from Kings College London. Erin loves the dynamic nature of her job, developing new extraction methods, and helping to design new analytical systems that have a profound influence on illnesses like diabetes and Alzheimer’s disease. She loves to play with her kids and being outdoors. Her favorite authors are Lincoln Child and Douglas Preston.
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