Peptide and Protein Bioanalysis Boot Camp

Building Proficiency in LC-MS Large Molecule Quantification

Peptide and Protein Bioanalysis Boot Camp

Waters’ team of bioanalysis experts developed the Peptide & Protein Bioanalysis Boot Camp – a series of short, recorded presentations – to help you and your colleagues acquire a fundamental and practical proficiency in large molecule quantification.

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Introduction to Peptide & Protein Bioanalysis Boot Camp

Quantifying large molecules in matrix is new to many of us and presents a variety of challenges that we don’t see with small molecules. Peptides and proteins have a more diverse set of chemical properties and tend to be much more complex. Kelly Doering, Director, Discovery & DMPK, explains the goals of the Peptide and Protein Bioanalysis Bootcamp.
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Introduction to Peptides and Proteins for Bioanalysis Using LC-MS

Khalid Khan, Senior Manager Business Development, discusses the basic structure of amino acids, peptides, and proteins, including specific examples like monoclonal antibodies. The ionization and fragmentation of peptides and how they differ from small molecules is also discussed.
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Contrasting LBAs and LC-MS for Peptide and Protein Bioanalysis

Kelly Doering discusses ligand binding assays (LBAs) and compares the advantages and limitations of this technology to LC-MS. She'll then present three examples of when LC-MS is the preferred method for quantification of insulin analogs based on specificity requirements and the need to multiplex.
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LC-MS/MS for Bioanalytical Peptide and Protein Quantification: MS Considerations

Caitlin Dunning, Associate Scientist, discusses how to use mass spectrometry to develop sensitive, selective, and robust quantification methods for peptide and protein quantification, including: basic mass spectrometry considerations, MS tuning, and best practices for success. In addition, practical considerations are provided to achieve greater MRM specificity for your peptides of interest.
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LC-MS/MS for Bioanalytical Peptide and Protein Quantification: Chromatographic Considerations

Bioanalytical scientists are faced with developing robust, reliable, and sensitive methods. This is especially challenging when we deal with peptides and proteins. Mary Lame, Principal Applications Chemist, walks you through a basic LC screening method designed specifically with peptides in mind.
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LC-MS/MS for Bioanalytical Peptide and Protein Quantification: Peptide Level Sample Clean-up

Bioanalytical laboratories are continually pressed to attain increasing levels of performance and productivity in the development of robust, reliable, and sensitive methods for accurate quantification. The challenge is the high sensitivity quantification of peptides and proteins from complex biological matrices. Mary Lame, Principal Applications Chemist, presents the starting universal solid phase extraction protocol for therapeutic, endogenous, and tryptic peptides. The value of incorporating a mixed mode SPE strategy into the overall LC-MS quantitative workflow is demonstrated.
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