Peptide and Protein Bioanalysis Boot Camp
Building Proficiency in LC-MS Large Molecule Quantification
Waters’ team of bioanalysis experts developed the Peptide & Protein Bioanalysis Boot Camp – a series of short, recorded presentations – to help you and your colleagues acquire a fundamental and practical proficiency in large molecule quantification.
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Introduction to Peptide & Protein Bioanalysis Boot Camp
Quantifying large molecules in matrix is new to many of us and presents a variety of challenges that we don’t see with small molecules. Peptides and proteins have a more diverse set of chemical properties and tend to be much more complex. Kelly Doering, Director, Discovery & DMPK, explains the goals of the Peptide and Protein Bioanalysis Bootcamp.
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Dr. Kelly Doering leads a business team within Waters’ Global Marketing group that focuses on the promotion of mass spectrometry solutions to Discovery and DMPK labs. Prior to Waters, she worked in a variety of commercial roles including product management, business development and new product development. She earned her doctorate from the University of Connecticut, studying nutrient regulation of hepatic gene expression in mice and then completed a post-doctoral fellowship in the Department of Endocrinology at Boston Childrens Hospital, studying the effects of glycemic index on body composition, also in rodent models. Before graduate school, she worked in Nutrition Research within the Division of Preventive & Behaviorial Medicine at UMass Medical Center, where she worked on a number of NIH funded projects, mainly centered around lifestyle interventions to improve cardiovascular outcomes.
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Introduction to Peptides and Proteins for Bioanalysis Using LC-MS
Khalid Khan, Senior Manager Business Development, discusses the basic structure of amino acids, peptides, and proteins, including specific examples like monoclonal antibodies. The ionization and fragmentation of peptides and how they differ from small molecules is also discussed.
- Access additional resources on this topic below:
- Simplifying Peptide Bioanalysis
- Generic Protein and Peptide Level Sample Preparation for Protein...
Khalid Khan is a senior manager with Waters Global Marketing, focusing on clinical and biomedical research applications. He has been involved in mass spectrometry technology for over 25 years since his postgraduate research studies where high resolution GC-MS data played a key role in the identification of novel organic compounds. Khalid joined Waters in 2001 and has been involved in both technical and business development roles in clinical diagnostics, toxicology and biomedical research applications of LC-MS. Khalid is currently focused on biomarker translation and quantification, with a particular interest in utilizing LC-MS in combination with advanced informatics to aid protein and peptide quantification to help advance biomedical research and drug development.
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Contrasting LBAs and LC-MS for Peptide and Protein Bioanalysis
Kelly Doering discusses ligand binding assays (LBAs) and compares the advantages and limitations of this technology to LC-MS. She'll then present three examples of when LC-MS is the preferred method for quantification of insulin analogs based on specificity requirements and the need to multiplex.
Dr. Kelly Doering leads a business team within Waters’ Global Marketing group that focuses on the promotion of mass spectrometry solutions to DMPK/Bioanalysis labs. Prior to Waters, she worked in a variety of commercial roles including product management, business development and new product development. She earned her doctorate from the University of Connecticut, studying nutrient regulation of hepatic gene expression in mice and then completed a post-doctoral fellowship in the Department of Endocrinology at Boston Childrens Hospital, studying the effects of glycemic index on body composition, also in rodent models. Before graduate school, she worked in Nutrition Research within the Division of Preventive & Behaviorial Medicine at UMass Medical Center, where she worked on a number of NIH funded projects, mainly centered around lifestyle interventions to improve cardiovascular outcomes.
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LC-MS/MS for Bioanalytical Peptide and Protein Quantification: MS Considerations
Caitlin Dunning, Associate Scientist, discusses how to use mass spectrometry to develop sensitive, selective, and robust quantification methods for peptide and protein quantification, including: basic mass spectrometry considerations, MS tuning, and best practices for success. In addition, practical considerations are provided to achieve greater MRM specificity for your peptides of interest.
Caitlin received her bachelor’s in Chemistry from Northeastern University (Boston, MA) where she participated in their signature cooperative education program. Leveraging her co-op experiences into a full-time position, Caitlin started her career in the field of proteomics. In this position, she learned that she loved bioanalytical chemistry, especially mass spectrometry. Caitlin joined Waters in 2016 as an Associate Applications Scientist where she provides support for new product development and discovery bioanalysis of proteins and peptides. She is responsible for all aspects of method development, including sample preparation, liquid chromatography, and mass spectrometry. She enjoys working on solutions to challenging bioanalytical applications and sharing her work with customers.
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LC-MS/MS for Bioanalytical Peptide and Protein Quantification: Chromatographic Considerations
Bioanalytical scientists are faced with developing robust, reliable, and sensitive methods. This is especially challenging when we deal with peptides and proteins. Mary Lame, Principal Applications Chemist, walks you through a basic LC screening method designed specifically with peptides in mind.
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- A Flexible SPE/LC/MS/MS Platform for the Simultaneous Quantitatio...
- Development of a High Sensitivity SPE-LC-MS/MS Assay for the Quan...
- LC-MS/MS Quantification of Intact Insulin-Like Growth Factor I (I...
Mary received her B.S. in Chemistry from the Western Connecticut State University and her M.S. in Chemistry from Central Connecticut State University. She began her bioanalytical career in 1997 with a global Pharmaceutical company where she supported research and discovery efforts by performing DMPK studies and conducting various bioanalytical assays until 2011. Mary joined Waters in 2011, providing pharmaceutical applications development and support for the Waters Scientific Operations Group. Mary’s primary role is to support discovery bioanalysis, and develop bioanalytical methods for drug molecules, both large and small. She is responsible for sample preparation, mass spectrometry, and LC method development.
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LC-MS/MS for Bioanalytical Peptide and Protein Quantification: Peptide Level Sample Clean-up
Bioanalytical laboratories are continually pressed to attain increasing levels of performance and productivity in the development of robust, reliable, and sensitive methods for accurate quantification. The challenge is the high sensitivity quantification of peptides and proteins from complex biological matrices. Mary Lame, Principal Applications Chemist, presents the starting universal solid phase extraction protocol for therapeutic, endogenous, and tryptic peptides. The value of incorporating a mixed mode SPE strategy into the overall LC-MS quantitative workflow is demonstrated.
Mary received her B.S. in Chemistry from the Western Connecticut State University and her M.S. in Chemistry from Central Connecticut State University. She began her bioanalytical career in 1997 with a global Pharmaceutical company where she supported research and discovery efforts by performing DMPK studies and conducting various bioanalytical assays until 2011. Mary joined Waters in 2011, providing pharmaceutical applications development and support for the Waters Scientific Operations Group. Mary’s primary role is to support discovery bioanalysis, and develop bioanalytical methods for drug molecules, both large and small. She is responsible for sample preparation, mass spectrometry, and LC method development.
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