Small Molecule Bioanalysis

When searching for the hard-to-find molecule, gaining insights and expertise from the DMPK community is critical. Browse these resources to be even more effective in quantifying small molecules for drug efficacy.

High Throughput Microflow LC-MS: Sensitivity Gains on a Practical Timescale

The sensitivity gains observed when using microflow LC-MS do not always translate into an everyday workflow advantage in a high throughput laboratory. The longer run times that are typical on a microflow scale deliver improved sensitivity, but decreased sample throughput when compared to UPLC. The ability to deliver high sensitivity at run times under 3 minutes compelled the need to develop a microflow device that can function on a UPLC timescale (<3 min).

This white paper introduces a novel microfluidic device capable of higher pressures and flow rates which enable run times under 3 minutes while still delivering improved sensitivity over traditional UPLC systems.

Analysis of Aldosterone Using a Highly Analytically Sensitive Tandem Quadrupole MS for Clinical Research

Presented at ASMS 2016, this poster demonstrates that the performance of UPLC coupled to a newly-developed tandem quadrupole MS has been shown to provide quantification down to 2 pg/mL aldosterone using only 200 µL of a serum sample.

Microflow LC-MS Application Notebook

This applications notebook contains application notes and white papers featuring ionKey/MS and the iKey Separation device.

See how we are bringing microflow LC-MS to the masses by creating a technology that standardizes and simplifies microflow. These application notes and whitepapers show how to increase sensitivity up to 40X, while reducing sample requirements, and saving up to 60X on solvents - which increases your confidence, saves you time and resources.

Acquiring and Processing Full Scan or Tof-MRM (Targeted HRMS) Datasets for Quantitative Assays

HRMS data for quantitative applications are becoming more and more widely accepted. UNIFI software enables collection of multiple modes of data and flexible quantitation workflows. UNIFI enables users to develop, process, and analyze HRMS bioanalysis assays with workflows that fit traditional tandem discovery or regulated bioanalytical workflows.

Tof-HRMS: Rescuing Low-Exposure Bioanalysis Applications from Chemical Noise

This blog discusses a hot topic - HRMS used in a regulated bioanalysis environment. Learn how GSK saw a 50-fold increase in assay sensitivity through the use of a SYNAPT G2-Si High Definition Mass Spectrometer to separate matrix interferences observed when using a unit resolution triple quadrupole MS.

What Sensitivity Gains can be Achieved by Combining Microflow LC with HRMS?

Using samples in human plasma, sub-femtogram on-column sensitivity is demonstrated using the ionKey/MS System with the Xevo G2-XS QTof.

QTof v. Triple Quad: in vivo Quantitation and Metabolite Identification of Plasma Samples

Advances in sensitivity, detector linear response, and robustness have opened up the possibility for QTof MS platforms to be a viable alternative for use in bioanalysis laboratories. This application note summarizes the results of a quant/qual workflow to determine robustness and reliability of QTof measurements, and compare them against the gold-standard tandem quadrupole workflows.

Ready for Prime Time: the Evolution of Microflow LC-MS

Capillary LC (cLC) coupled to MS has the potential to improve detection limits, address limited sample volumes and allow multiple analyses from one sample. This is particularly attractive in areas where ultrahigh assay sensitivity, low limits of detection and small sample volumes are becoming commonplace. The authors review the evolution of microscale LC/MS in DMPK, bioanalysis, metabolic profiling, and biofluid analysis.

HRMS and Microflow LC Combine for Excellent Sensitivity, Linearity, Peak Shape, and Resolution

Bioanalysis to support PK-PD studies and clinical trials can be challenging especially when quantifying highly potent compounds. In this study, quantitation attributes were determined for a set of drug-like compounds in human plasma using microflow LC and high resolution mass spectrometry (HRMS) with ionKey/MS and the Xevo G2-XS Time of Flight (Tof) mass spectrometer.

How Bioanalysts Use and Evaluate the Future of LC-MS

Bioanalysis Zone surveyed scientists from all over the world across different types and sizes of organizations to better understand how bioanalysts apply LC-MS methods in their respective fields and how it may change techniques and approaches in the future.

Achieving 15-30 Fold Improvement in Sensitivity Utilizing Microfluidics for Peptide Quantification

Critical bioanalytical assays were adapted to integrated microscale LC to reduce sample volumes while increasing sensitivity. Assays for teriparatide, glucagon and human insulin and five analogs were transferred from 2.1 mm analytical scale LC to a 150 µm scale system.

What's New? Microfluidic Separation Devices Coupled to MS

In this publication, the utilization of chip-based separation devices coupled to MS for analyzing peptides/proteins, glycans, drug metabolites and biomarkers for various bioanalytical applications are evaluated.

The Ever-Expanding Role of HRMS in Regulated Bioanalysis

Dr. Chris Evans (Director Bioanalytical Sciences and Toxicokinetics, GSK) showcases case studies where HRMS facilitated adaptable bioanalytical study support to: overcome challenges associated with selectivity; understand molecular integrity; and facilitate intact analysis.

Achieving Femtogram-level On-column Sensitivity for Quantitation of Drugs in Human Plasma

Advances in HRMS have led to improved selectivity, dynamic range, linearity, and sensitivity, In the presence of complex matrix interferences that might prove challenging for triple quadrupole MS approaches, the markedly different nature of HRMS data acquisition can achieve the selectivity and sensitivity for successful quantitation.

High Throughput Analysis with Microfluidics Under Gradient Conditions

The ionKey/MS System with Xevo G2-XS Qtof HRMS is a high-performance platform that can be used for highly sensitive detection of small molecules, peptides, and proteins. This application shows the ionKey/MS can also be operated under high throughput conditions with a 3-minute full gradient and a 7 μL/min flow rate.

Exploring the Versatility of Microflow Technology from Peptide Biomarkers to Small Molecules

The use of microfluidic technology for peptide quantitation yields the same or better sensitivity when compared to a nanoscale platform and has the additional, very significant advantages of ease of use, robustness, and improved chromatographic resolution (e.g. peak capacity). Thus, with a single analytical platform we can perform quantitative analysis for a wide range of compounds spanning from small molecules to peptides.

Is Ion Mobility Mass Spectrometry Child's Play?

Waters is making ion mobility as easy to use as your cell phone or your remote control. Take a look at this blog describing the merits of ion mobility for routine mass spectrometry.

Bridging the Gap…Connecting Evolving Bioanalysis Expertise to a Rapidly Changing Global Biopharma Industry

As the pharmaceutical industry continues to shift more toward biologics and hybrid drug entities, it becomes increasingly imperative that the small and large molecule bioanalysis worlds work together to efficiently arrive at go/no-go decisions. This webinar will address these trends as well as the impact on drug development and regulatory requirements for drug approval.

Are Analytical Chemists Going Extinct?

In this blog, the idea of an 'endangered' analytical chemists is explored. They face a myriad of obstacles today – lower than average job forecasts, costly and timely training, demand for more versatile knowledge, tighter regulations, and massive data management requirements. What do you think?