Small Molecule Bioanalysis

When searching for the hard-to-find molecule, gaining insights and expertise from the DMPK community is critical. Browse these resources to be even more effective in quantifying small molecules for drug efficacy.

A Quantitative UPLC-MS/MS Research Method for the Measurement of Acetaminophen and 5 Metabolites in Plasma

This application note describes a sensitive, validated, UPLC-based bioanalytical research method for the quantification of acetaminophen and five metabolites in plasma.

The research method uses reversed-phase UPLC to obtain suitable chromatographic properties, such as retention of the analytes and the resolution of metabolites, with a run time of 7.5 min. A quantitative method was validated over the range 16 ng/mL–500 ng/mL for acetaminophen, 3.2 ng/mL–100 ng/mL for both acetaminophen glucuronide and sulfate, 0.64 ng/mL–20 ng/mL for acetaminophen cysteinyl and glutathione metabolite and 0.96 ng/mL–20 ng/mL for acetaminophen N-acetyl cysteinyl metabolite. The methodology required only 5 μL of plasma and exhibited excellent sensitivity, robustness and reproducibility.

Data Independent and Data Dependent Acquisition Strategies Combined With Ion Mobility for Drug Metabolism and Pharmacokinetics

This poster presented at 12th Annual Great Lakes DMDG meeting from May 4-5th in Michigan.

An HS-MRM Assay for Quantification of Host Cell Proteins in Therapeutic Monoclonal Antibodies

This poster presented at 65th ASMS Conference from June 4-8 in Indianapolis, IN.

A Sensitive Microflow LC/MS/MS Method for the Analysis of Fluticasone Propionate in Human Plasma

This poster presented at 65th ASMS Conference from June 4-8 in Indianapolis, IN.

Building a Collision Cross Section Library of Pharmaceutical Drugs Using an IMS QTof Platform

This poster presented at 65th ASMS Conference from June 4-8 in Indianapolis, IN.

High Throughput Microflow LC-MS: Sensitivity Gains on a Practical Timescale

The sensitivity gains observed when using microflow LC-MS do not always translate into an everyday workflow advantage in a high throughput laboratory. The longer run times that are typical on a microflow scale deliver improved sensitivity, but decreased sample throughput when compared to UPLC. The ability to deliver high sensitivity at run times under 3 minutes compelled the need to develop a microflow device that can function on a UPLC timescale (<3 min).

This white paper introduces a novel microfluidic device capable of higher pressures and flow rates which enable run times under 3 minutes while still delivering improved sensitivity over traditional UPLC systems.

Analysis of Aldosterone Using a Highly Analytically Sensitive Tandem Quadrupole MS for Clinical Research

Presented at ASMS 2016, this poster demonstrates that the performance of UPLC coupled to a newly-developed tandem quadrupole MS has been shown to provide quantification down to 2 pg/mL aldosterone using only 200 µL of a serum sample.

Tof-HRMS: Rescuing Low-Exposure Bioanalysis Applications from Chemical Noise

This blog discusses a hot topic - HRMS used in a regulated bioanalysis environment. Learn how GSK saw a 50-fold increase in assay sensitivity through the use of a SYNAPT G2-Si High Definition Mass Spectrometer to separate matrix interferences observed when using a unit resolution triple quadrupole MS.

Microflow LC-MS Application Notebook

This applications notebook contains application notes and white papers featuring ionKey/MS and the iKey Separation device.

See how we are bringing microflow LC-MS to the masses by creating a technology that standardizes and simplifies microflow. These application notes and whitepapers show how to increase sensitivity up to 40X, while reducing sample requirements, and saving up to 60X on solvents - which increases your confidence, saves you time and resources.

Acquiring and Processing Full Scan or Tof-MRM (Targeted HRMS) Datasets for Quantitative Assays

HRMS data for quantitative applications are becoming more and more widely accepted. UNIFI software enables collection of multiple modes of data and flexible quantitation workflows. UNIFI enables users to develop, process, and analyze HRMS bioanalysis assays with workflows that fit traditional tandem discovery or regulated bioanalytical workflows.