Large Molecule Quantification

Address the quantification of peptide- and protein-based biotherapeutics and biomarkers.

A Leading CRO's Customer Focus Leads to Improvements in Quality and Productivity

Triskelion, a TNO company, demonstrates how they provide leading edge quality through a combination of service, skills, and technology.

Peptide Optimization Using Skyline and the Xevo TQ-XS

This technology brief demonstrates the workflow for peptide MRM optimization using the Xevo TQ-XS Triple Quadrupole Mass Spectrometer, Skyline software and phosphorylation B peptide standard.

Why Should Bioanalysts Use Microflow LC-MS?

Why are scientists at leading companies like GlaxoSmithKline, Merck, and Pfizer using microflow LC-MS for more routine applications? The answer is simple: an overarching need to do more with less. They are working with more complex analytes, with limited sample, often requiring both improved sensitivity and high throughput. Although microflow LC-MS has been perceived as a tradeoff between high sensitivity and throughput, recent advances are yielding flexible systems that enable analysts to collect highly sensitive data with cycle times comparable to analytical scale.

Sensitive and Reproducible LC-MS Quantification of C-Reactive Protein in Plasma: A Potential Biomarker of Inflammation

This application note demonstrates accurate, reproducible quantification of endogenous CRP levels in plasma using a generic kit-based approach.

Peptide Separations Application Notebook

This document is a compilation of published LC and LC-MS applications for the analyses of peptides.

Quantitation of Biopharmaceuticals by Mass Spectrometry

The quantitation of proteins in complex biological samples is an important and challenging task. Quantitation of small molecules, while less challenging, also requires some special considerations. This new e-book presents essential information about these analyses, from general precepts through specific considerations when developing an LC-MS-MS method.

Application of High- and Low-resolution Mass Spectrometers Combined with Nanoscale LC and Microfluidic LC Platforms for the Quantitative Analysis of Peptide Biomarkers in Human Serum

This application note presents the comprehensive comparison of both tandem quadrupole and time-of-flight mass spectrometers for the quantitative MRM-based analysis of peptide biomarkers.

The microfluidic LC platform combined with the Xevo TQ-S tandem quadrupole mass spectrometer is the ideal LC-MS system for translational research studies where low level quantitation of tryptic peptides is required.

Improvements in Sensitivity for Biotherapeutics Using a Tandem Quadrupole MS

Presented at ASMS 2016, this poster presents data that was generated using a prototype tandem quadrupole MS. Oxytocin, Vancomycin (cyclic peptides), and a large monoclonal antibody were selected as candidate molecules to cover a cross-section of analytically-challenging biotherapeutics.

Microflow LC-MS Application Notebook

This applications notebook contains application notes and white papers featuring ionKey/MS and the iKey Separation device.

See how we are bringing microflow LC-MS to the masses by creating a technology that standardizes and simplifies microflow. These application notes and whitepapers show how to increase sensitivity up to 40X, while reducing sample requirements, and saving up to 60X on solvents - which increases your confidence, saves you time and resources.

Quantification of Intact Protein Biotherapeutics by a Microfluidic LC-MS Platform Poster

This poster aims to evaluate the analytical performance of the ionKey/Xevo G2-XS system for quantification of protein therapeutics at the intact level.